Abstract
Genetically distinct forms of Pneumocystis carinii infect several mammalian hosts. We report the amplification of P. carinii DNA from samples of two infected thoroughbred foal lungs by using primers designed from the sequence of a P. carinii mitochondrial rRNA gene; these primers also prime the amplification of P. carinii DNA from other hosts. The nucleotide sequence of part of the mitochondrial rRNA gene amplified from P. carinii infecting one of the foals was determined and found to be distinct from that of published rat-, rabbit-, ferret-, and human-derived P. carinii sequences.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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DNA Primers
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DNA, Mitochondrial / genetics
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DNA, Ribosomal / genetics
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Female
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Ferrets
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Gene Amplification
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Horse Diseases / microbiology*
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Horses
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Humans
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Lung / microbiology
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Male
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Mice
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Molecular Sequence Data
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Pneumocystis / classification
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Pneumocystis / genetics*
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Pneumonia, Pneumocystis / genetics
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Pneumonia, Pneumocystis / microbiology
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Pneumonia, Pneumocystis / veterinary*
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Rabbits
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Rats
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Sequence Homology, Nucleic Acid
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Species Specificity
Substances
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DNA Primers
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DNA, Mitochondrial
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DNA, Ribosomal