Reduction of disulfide bonds in an amyloidogenic Bence Jones protein leads to formation of "amyloid-like" fibrils in vitro

Biol Chem Hoppe Seyler. 1993 Dec;374(12):1117-22. doi: 10.1515/bchm3.1993.374.7-12.1117.

Abstract

Motivated by the finding that the amino acid sequence of the Bence Jones protein BJP-DIA was identical to that of the main protein component of the amyloid fibrils obtained from the same patient with AL-amyloidosis, (Klafki, H.-W., Kratzin, H.-D., Pick, A.-I., Eckart, K., Karas, M. & Hilschmann, N. (1992) Biochemistry 31, 3265-3272.), we attempted to create "amyloid-like" fibrils from the Bence Jones protein in vitro, without addition of proteolytic enzymes. Reduction of BJP-DIA, solubilized in PBS, pH 7.4, overnight at 37 degrees C resulted in the formation of a precipitate which had affinity for the dye Congo red. Electron microscopy of negatively stained samples of the reduced protein revealed aggregates of linear unbranched fibrils. SDS-polyacrylamide gel electrophoresis demonstrated that the precipitate consisted almost exclusively of intact light chain molecules. This result makes it possible to deduce a molecular model of these amyloid fibrils generated in vitro.

MeSH terms

  • Amyloid / biosynthesis*
  • Bence Jones Protein / chemistry*
  • Bence Jones Protein / isolation & purification
  • Bence Jones Protein / ultrastructure
  • Chromatography, Ion Exchange
  • Disulfides / chemistry*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Microscopy, Electron
  • Oxidation-Reduction

Substances

  • Amyloid
  • Disulfides
  • Bence Jones Protein