Abstract
Most of the translocations affecting the chromosome band 11q23, frequently seen in human acute leukemias, involve a restricted area of the HRX gene. We have characterized two t(1;11)(p32;q11) translocations which fuse the HRX gene to a novel gene, AF-1p on chromosome 1p32, in two myeloid leukemias. The der (11) chromosome expresses the 1368 N-terminal amino acids of HRX, including the AT-hook, snRNP and methyltransferase similarities, fused to almost all the AF-1p product. The predicted wild type AF-1p product is a 98 kDa acidic protein which does not exhibit similarity to the AF-4, AF-9 and ENL gene products. It is highly similar to the murine eps 15 gene product, which encodes a cytoplasmic phosphoprotein. Our data indicate that AF-1p defines another class of genes fused to HRX in 11q23 abnormalities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Adult
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Calcium-Binding Proteins*
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Child, Preschool
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Chromosomes, Human, Pair 1*
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Chromosomes, Human, Pair 11*
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Cloning, Molecular
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Consensus Sequence
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DNA-Binding Proteins / genetics*
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Female
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Histone-Lysine N-Methyltransferase
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Humans
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Intracellular Signaling Peptides and Proteins
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Leukemia, Monocytic, Acute / genetics*
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Leukemia, Myeloid / genetics*
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Male
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Molecular Sequence Data
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Myeloid-Lymphoid Leukemia Protein
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Phosphoproteins*
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Polymerase Chain Reaction
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Proto-Oncogenes*
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Restriction Mapping
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Transcription Factors*
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Translocation, Genetic*
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Zinc Fingers
Substances
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Adaptor Proteins, Signal Transducing
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Calcium-Binding Proteins
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DNA-Binding Proteins
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EPS15 protein, human
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Intracellular Signaling Peptides and Proteins
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KMT2A protein, human
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Phosphoproteins
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Transcription Factors
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Myeloid-Lymphoid Leukemia Protein
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Histone-Lysine N-Methyltransferase
Associated data
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GENBANK/Z29064
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GENBANK/Z29070