Abstract
The response of a cell line of Xenopus laevis melanophores to serotonin was examined. Serotonin increased intracellular levels of cAMP and induced pigment dispersion in the cells. The responses depended on both the concentration of serotonin applied and on the time for which the cells were exposed to serotonin. Using a recently described, microtiter-plate-based bioassay, a series of serotonin receptor ligands were evaluated as agonists or antagonists at the melanophore serotonin receptor. The pharmacological profile suggests the presence of a receptor which shares some properties with but appears different from other previously described serotonin receptors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cells, Cultured
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Cyclic AMP / metabolism
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Intracellular Fluid / metabolism
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Melanophores / drug effects
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Melanophores / metabolism
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Melanophores / ultrastructure*
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Pigments, Biological / metabolism
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Receptors, Serotonin / drug effects
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Receptors, Serotonin / physiology*
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Second Messenger Systems / drug effects
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Second Messenger Systems / physiology
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Serotonin / pharmacology
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Serotonin Antagonists
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Serotonin Receptor Agonists / pharmacology
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Xenopus laevis
Substances
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Pigments, Biological
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Receptors, Serotonin
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Serotonin Antagonists
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Serotonin Receptor Agonists
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Serotonin
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Cyclic AMP