Abstract
Novel flexible oligodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidite approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications in the middle or one or two modifications in the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modified oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Enzyme Stability
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Magnetic Resonance Spectroscopy
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Molecular Sequence Data
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Molecular Structure
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Nucleic Acid Hybridization
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Oligodeoxyribonucleotides / chemistry*
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Oligodeoxyribonucleotides / metabolism
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Phosphodiesterase I
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Phosphoric Diester Hydrolases / metabolism
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Thymidine / analogs & derivatives
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Thymidine / chemistry*
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Thymidine / metabolism
Substances
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Oligodeoxyribonucleotides
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Phosphoric Diester Hydrolases
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Phosphodiesterase I
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Thymidine