Purification of Chlamydia trachomatis strains in mixed infection by monoclonal antibody neutralization

J Clin Microbiol. 1994 Feb;32(2):533-5. doi: 10.1128/jcm.32.2.533-535.1994.

Abstract

A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster kidney cells, the D* variant strain was enriched until it could be cloned by limiting dilution. This newly described neutralization enrichment procedure can be used to purify a C. trachomatis serovar present in small numbers in a mixed culture or, potentially, to identify nonneutralizable mutants.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / immunology
  • Base Sequence
  • Cells, Cultured
  • Chlamydia Infections / microbiology*
  • Chlamydia trachomatis / genetics
  • Chlamydia trachomatis / immunology*
  • Chlamydia trachomatis / isolation & purification*
  • Cricetinae
  • DNA, Bacterial / genetics
  • Female
  • Genes, Bacterial
  • Genetic Variation
  • Humans
  • Molecular Sequence Data
  • Neutralization Tests
  • Porins*
  • Serotyping

Substances

  • Antibodies, Monoclonal
  • Bacterial Outer Membrane Proteins
  • DNA, Bacterial
  • Porins
  • omp1 protein, Chlamydia trachomatis