Abstract
A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster kidney cells, the D* variant strain was enriched until it could be cloned by limiting dilution. This newly described neutralization enrichment procedure can be used to purify a C. trachomatis serovar present in small numbers in a mixed culture or, potentially, to identify nonneutralizable mutants.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal*
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / immunology
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Base Sequence
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Cells, Cultured
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Chlamydia Infections / microbiology*
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Chlamydia trachomatis / genetics
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Chlamydia trachomatis / immunology*
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Chlamydia trachomatis / isolation & purification*
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Cricetinae
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DNA, Bacterial / genetics
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Female
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Genes, Bacterial
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Genetic Variation
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Humans
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Molecular Sequence Data
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Neutralization Tests
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Porins*
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Serotyping
Substances
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Antibodies, Monoclonal
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Bacterial Outer Membrane Proteins
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DNA, Bacterial
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Porins
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omp1 protein, Chlamydia trachomatis