Administration of amyloid beta-peptides into the medial septum of rats decreases acetylcholine release from hippocampus in vivo

E Abe; F Casamenti; L Giovannelli; C Scali; G Pepeu

doi:10.1016/0006-8993(94)90193-7

Administration of amyloid beta-peptides into the medial septum of rats decreases acetylcholine release from hippocampus in vivo

Brain Res. 1994 Feb 4;636(1):162-4. doi: 10.1016/0006-8993(94)90193-7.

Authors

E Abe¹, F Casamenti, L Giovannelli, C Scali, G Pepeu

Affiliation

¹ Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.

PMID: 8156403
DOI: 10.1016/0006-8993(94)90193-7

Abstract

The septum of male Wistar rats was injected with synthetic beta-amyloid fragments, beta 12-28, beta 25-35 and beta 1-40, and hippocampal acetylcholine (ACh) release was evaluated by transversal microdialysis. A marked decrease in basal and K(+)-evoked ACh release was found 7 or 21 days after injection of 5 nmol of beta 12-28 and beta 25-35, or 3 nmol of beta 1-40, respectively. These data indicate that septal injection of beta-amyloid peptides causes hypofunction of the septo-hippocampal cholinergic system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylcholine / metabolism*
Amyloid beta-Peptides / administration & dosage
Amyloid beta-Peptides / pharmacology*
Animals
Brain
Choline O-Acetyltransferase / metabolism
Congo Red
Hippocampus / drug effects
Hippocampus / metabolism*
Histocytochemistry
Injections
Male
Microdialysis
Parasympathetic Nervous System / cytology
Parasympathetic Nervous System / drug effects
Peptide Fragments / pharmacology
Potassium / pharmacology
Rats
Rats, Wistar

Substances

Amyloid beta-Peptides
Peptide Fragments
Congo Red
Choline O-Acetyltransferase
Acetylcholine
Potassium