Competitive in situ hybridization in a mediastinal germ cell tumor

M S Aly; P Dal Cin; P Jiskoot; G Deneffe; P Marynen; H Van den Berghe

doi:10.1016/0165-4608(94)90181-3

Competitive in situ hybridization in a mediastinal germ cell tumor

Cancer Genet Cytogenet. 1994 Mar;73(1):53-6. doi: 10.1016/0165-4608(94)90181-3.

Authors

M S Aly¹, P Dal Cin, P Jiskoot, G Deneffe, P Marynen, H Van den Berghe

Affiliation

¹ Center for Human Genetics, University Hospital Gasthuisberg, Belgium.

PMID: 8174074
DOI: 10.1016/0165-4608(94)90181-3

Abstract

We performed competitive in situ hybridization in a mediastinal germ cell tumor to identify chromosome 12 aberration(s). DNA from a mouse-human somatic hybrid cell line, containing an i(12p) as unique human material, was labeled and used as a probe. Our results confirm that the chromosome marker, observed in mediastinal germ cell tumor and cytogenetically identified as i(12p), is a true isochromosome of the short arm of chromosome 12.

Publication types

Case Reports
Review

MeSH terms

Adolescent
Animals
Chromosome Aberrations*
Chromosome Banding
Chromosome Disorders*
Chromosomes, Human, Pair 12*
Germinoma / genetics*
Germinoma / pathology
Germinoma / therapy
Humans
Hybrid Cells
In Situ Hybridization* / methods
Male
Mediastinal Neoplasms / genetics*
Mediastinal Neoplasms / pathology
Mediastinal Neoplasms / therapy
Mice