Prokaryotic expression of a large fragment of the most antigenic cytomegalovirus DNA-binding protein (ppUL44) and its reactivity with human antibodies

A Ripalti; P Dal Monte; M C Boccuni; F Campanini; T Lazzarotto; B Campisi; Q Ruan; M P Landini

doi:10.1016/0166-0934(94)90015-9

Prokaryotic expression of a large fragment of the most antigenic cytomegalovirus DNA-binding protein (ppUL44) and its reactivity with human antibodies

J Virol Methods. 1994 Jan;46(1):39-50. doi: 10.1016/0166-0934(94)90015-9.

Authors

A Ripalti¹, P Dal Monte, M C Boccuni, F Campanini, T Lazzarotto, B Campisi, Q Ruan, M P Landini

Affiliation

¹ Department of Microbiology, School of Medicine, St. Orsola General Hospital, University of Bologna, Italy.

PMID: 8175946
DOI: 10.1016/0166-0934(94)90015-9

Abstract

We isolated and characterized from a lambda gt11 expression library clones expressing portions of human cytomegalovirus (HCMV)-p52. This nonstructural viral protein is encoded by UL44 and is known to be one of the best IgM reactive antigens. The reactivity of these clones was studied with human antibody and the gene fragment coding for the most immune-reactive portion of p52 (aa 202-434) was cloned in a prokaryotic expression vector, pROS, which overexpresses the antigen as a fusion protein to a truncated molecule of beta-galactosidase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / immunology*
Antigens, Viral / immunology*
Cytomegalovirus / genetics*
Cytomegalovirus / immunology
DNA-Binding Proteins / biosynthesis*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / immunology
Humans
Immunoglobulin M / immunology*
Recombinant Fusion Proteins / biosynthesis*
Recombinant Fusion Proteins / immunology
Viral Proteins / biosynthesis*
Viral Proteins / genetics
Viral Proteins / immunology

Substances

Antibodies, Viral
Antigens, Viral
DNA-Binding Proteins
ICP36 protein, Cytomegalovirus
Immunoglobulin M
Recombinant Fusion Proteins
Viral Proteins