The in vitro activity of teicoplanin and ramoplanin against 113 Clostridium difficile strains isolated from different origins was determined. The minimum inhibitory concentrations (MICs) of antibiotics were determined by the agar dilution method. MIC of teicoplanin and ramoplanin were twofold lower for Clostridium difficile strains isolated from patient with pseudomembranous colitis. No correlation between the origin of the strains toxigenicity and susceptibility to teicoplanin and ramoplanin was observed. Our result indicate that teicoplanin and ramoplanin are potentially effective alternative antibiotics for treatment of infections caused by Clostridium difficile.