Analysis of the 22 kbp long psbD-psbC gene cluster of Euglena gracilis chloroplast DNA: evidence for overlapping transcription units undergoing differential processing

B Orsat; A Spielmann; S Marc-Martin; T Lemberger; E Stutz

doi:10.1016/0167-4781(94)90102-3

Analysis of the 22 kbp long psbD-psbC gene cluster of Euglena gracilis chloroplast DNA: evidence for overlapping transcription units undergoing differential processing

Biochim Biophys Acta. 1994 May 17;1218(1):75-81. doi: 10.1016/0167-4781(94)90102-3.

Authors

B Orsat¹, A Spielmann, S Marc-Martin, T Lemberger, E Stutz

Affiliation

¹ Laboratoire de Biochimie Végétale, Université de Neuchâtel, Switzerland.

PMID: 8193167
DOI: 10.1016/0167-4781(94)90102-3

Abstract

The clustered genes psbD and psbC covering together close to 22,000 nucleotides contain ten and eleven exons, respectively. The corresponding translation products, i.e, Photosystem II core 34 kDa (D2) protein and the CP43 chlorophyll binding protein are highly conserved. Introns vary in length from 305 to 4144 nucleotides. The two genes have about 900 nucleotides in common including an intron. To obtain stable mRNAs of about 1400 (psbD) and 1500 (psbC) nucleotides the pre-transcripts must undergo differential processing and/or splicing events within the overlapping region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Chloroplasts / metabolism*
Cloning, Molecular
DNA
Euglena gracilis / genetics*
Light-Harvesting Protein Complexes
Molecular Sequence Data
Multigene Family*
Photosynthetic Reaction Center Complex Proteins / genetics*
Photosystem II Protein Complex
RNA Processing, Post-Transcriptional*
RNA Splicing
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Alignment

Substances

Light-Harvesting Protein Complexes
Photosynthetic Reaction Center Complex Proteins
Photosystem II Protein Complex
RNA, Messenger
photosystem II, chlorophyll binding protein, CP-43
DNA

Associated data

GENBANK/X70810