Abstract
Erythroid Kruppel-like factor (EKLF) is an erythroid-specific transcription factor that binds a CACCC motif found in the human beta-globin gene promoter. We have studied the promoter of the EKLF gene and identified binding sites for the transcription factors GATA-1 and CCAAT-binding Protein 1 (CP1). We show that both types of binding sites are required for full activity, and that the GATA motif at -60 is essential. The EKLF promoter can be directly activated in nonerythroid cells in cotransfection experiments by forced expression of GATA-1. These results suggest that EKLF is dependent on GATA-1 for its expression and lies downstream of, or coincident with, GATA-1 in a regulatory hierarchy in erythroid development.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Base Sequence
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Binding Sites
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Cell Line
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Cloning, Molecular
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DNA Primers
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Gene Expression Regulation*
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Globins / genetics*
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Growth Hormone / genetics
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Humans
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Kruppel-Like Transcription Factors
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic*
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Restriction Mapping
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
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Transcription, Genetic
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Transfection
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Zinc Fingers / genetics
Substances
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DNA Primers
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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GATA1 protein, human
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Gata1 protein, mouse
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Kruppel-Like Transcription Factors
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Transcription Factors
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erythroid Kruppel-like factor
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Growth Hormone
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Globins