[Amplification of 16S rDNAs from halobacteria by means of PCR technique]

P Zhou; Y Xu; Y Ma; H Liu

[Amplification of 16S rDNAs from halobacteria by means of PCR technique]

Wei Sheng Wu Xue Bao. 1994 Feb;34(1):6-8.

[Article in Chinese]

Authors

P Zhou¹, Y Xu, Y Ma, H Liu

Affiliation

¹ Institute of Microbiology, Academia Sinica, Beijing.

PMID: 8209549

Abstract

The 16S rDNAs have been amplified from four halobacteria Haloarcula vallismortis (EM201), Haloferax denitrificans (EM303). A5 and B2 through a set of specific primers using the PCR technique. The molecule of the PCR products is about 1.47 kb. The hybridization results also show that these PCR products have DNA homology with halobacteria.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA, Bacterial / genetics*
Halobacteriaceae / classification
Halobacteriaceae / genetics*
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics*

Substances

DNA, Bacterial
RNA, Ribosomal, 16S