Abstract
A monoclonal antibody resistant (MAR) variant of MHV was isolated after infection of hybridoma cells secreting the neutralizing and fusion-inhibiting monoclonal antibody, mAb 11F. The isolated variant was able to mediate syncytia formation even in the presence of high concentrations of mAb 11F. The S gene of the variant was cloned and sequenced. There were three nucleotide exchanges in comparison to the wild-type S gene, resulting in two amino acid alterations. However, both amino acid substitutions (at positions 255 and 1116) were located outside the binding site of mAb 11F.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology*
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Antibodies, Viral / immunology*
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Astrocytoma
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Cell Fusion
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Cloning, Molecular
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Cytopathogenic Effect, Viral
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DNA Mutational Analysis
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DNA, Complementary / genetics
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Hybridomas
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L Cells / microbiology
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / metabolism
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Mice
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Molecular Sequence Data
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Murine hepatitis virus / genetics*
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Murine hepatitis virus / physiology
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Sequence Alignment
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Sequence Homology, Amino Acid
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Spike Glycoprotein, Coronavirus
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Tumor Cells, Cultured
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Viral Envelope Proteins / genetics*
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Viral Envelope Proteins / metabolism
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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DNA, Complementary
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Membrane Glycoproteins
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Spike Glycoprotein, Coronavirus
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Viral Envelope Proteins