Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes

P Delvenne; B Kaschten; J M Deneufbourg; L Demanez; A Stevenaert; M Reznik; J Boniver

doi:10.1007/BF01606589

Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes

Virchows Arch A Pathol Anat Histopathol. 1993;423(2):145-50. doi: 10.1007/BF01606589.

Authors

P Delvenne¹, B Kaschten, J M Deneufbourg, L Demanez, A Stevenaert, M Reznik, J Boniver

Affiliation

¹ Department of Pathology, University Hospital of Liège, Belgium.

PMID: 8212542
DOI: 10.1007/BF01606589

Abstract

A case of nasopharyngeal carcinoma is presented. Epstein-Barr viral genome was identified in the neoplastic cells by in situ hybridization with digoxigenin-labelled polymerase chain reaction-generated probes. We report the development of this technique in paraffin-embedded sections and propose that such identification may prove valuable for the diagnosis of this tumour in routine material.

Publication types

Case Reports

MeSH terms

Adult
Base Sequence
Carcinoma / microbiology*
Carcinoma / pathology
Digoxigenin
Herpesvirus 4, Human / genetics
Herpesvirus 4, Human / isolation & purification*
Humans
In Situ Hybridization*
Magnetic Resonance Imaging
Male
Molecular Probes / genetics
Molecular Sequence Data
Nasopharyngeal Neoplasms / microbiology*
Nasopharyngeal Neoplasms / pathology
Polymerase Chain Reaction

Substances

Molecular Probes
Digoxigenin