Abstract
Immunization of Balb/c mice with conjugates of oligosaccharide haptens and crystalline bacterial surface-layer proteins (S-layers) primed the mice for a strong, hapten-specific, delayed-type hypersensitivity (DTH) response. Conjugates of haptens with bovine serum albumin produced only weak DTH responses but, when mixed with aluminium hydroxide, elicited DTH responses comparable to those against S-layer conjugates. Surface-layer conjugates also elicited strong anti-hapten DTH responses when administered by an oral/nasal route. Apparently, the natural assembly of S-layer proteins into large, two-dimensional arrays endows them with intrinsic adjuvant properties.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic
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Administration, Oral
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Aluminum Hydroxide / immunology
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Animals
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Antigens / administration & dosage
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Antigens / immunology*
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Bacterial Proteins / immunology*
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Bacterial Proteins / isolation & purification
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Carbohydrate Sequence
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Cell Wall / chemistry
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Cell Wall / immunology
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Crystallization
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Cyclophosphamide / pharmacology
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Drug Carriers
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Female
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Haptens / administration & dosage
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Haptens / immunology
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Hypersensitivity, Delayed / immunology
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Immunity, Cellular / drug effects
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Immunization / methods*
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Immunotherapy, Adoptive
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Lymphocyte Activation / drug effects
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Mice
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Mice, Inbred BALB C / immunology
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Molecular Sequence Data
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Oligosaccharides / administration & dosage
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Oligosaccharides / immunology*
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Serum Albumin, Bovine / immunology
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T-Lymphocytes / immunology*
Substances
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Adjuvants, Immunologic
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Antigens
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Bacterial Proteins
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Drug Carriers
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Haptens
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Oligosaccharides
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Serum Albumin, Bovine
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Aluminum Hydroxide
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Cyclophosphamide