Method for the direct specific measurement of vitamin B12 bound to transcobalamin II in plasma

Acta Haematol. 1993;89(4):195-9. doi: 10.1159/000204522.

Abstract

The concentration of plasma vitamin B12 bound to transcobalamin II was measured by two methods: QUSO and Heparin Sepharose (HS). The new HS procedure provided a specific, direct measurement of vitamin B12 bound to both R binder and transcobalamin II in plasma.

Publication types

  • Comparative Study

MeSH terms

  • Cobalt Radioisotopes
  • Humans
  • Indicator Dilution Techniques
  • Indicators and Reagents
  • Protein Binding
  • Sepharose / analogs & derivatives
  • Silicon Dioxide
  • Transcobalamins / metabolism*
  • Vitamin B 12 / blood*

Substances

  • Cobalt Radioisotopes
  • Indicators and Reagents
  • Transcobalamins
  • heparin-sepharose
  • Silicon Dioxide
  • Sepharose
  • Vitamin B 12