Interaction between HMG proteins (1 + 2) and the negative regulatory region 1 (NCR1) in the 5'-flanking sequence of the human beta-globin gene

R L Qian; Y D Chen; Y L Hu; M Q Li; Z H Wang; Y L Xu

Interaction between HMG proteins (1 + 2) and the negative regulatory region 1 (NCR1) in the 5'-flanking sequence of the human beta-globin gene

Sci China B. 1993 Aug;36(8):933-9.

Authors

R L Qian¹, Y D Chen, Y L Hu, M Q Li, Z H Wang, Y L Xu

Affiliation

¹ Shanghai Institute of Cell Biology, Academia Sinica, PRC.

PMID: 8216745

Abstract

The pattern of high mobility group proteins 1 and 2 (HMG1,2) interaction with the 5'-flanking sequence of the human beta-globin gene has been analyzed by scanning tunnelling microscopy (STM). A 200 bp negative regulatory region in the 5'-flanking sequence of the human beta-globin gene can be folded by HMG proteins 1 and 2 into a circular structure (diameter 70 +/- 6 A) with a linear tail which seems to be a left-handed double helix structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding Sites
DNA / genetics
DNA / ultrastructure
Globins / genetics*
High Mobility Group Proteins / genetics*
High Mobility Group Proteins / metabolism
High Mobility Group Proteins / ultrastructure
Humans
Microscopy, Scanning Tunneling
Molecular Sequence Data
Protein Binding / genetics
Regulatory Sequences, Nucleic Acid*

Substances

High Mobility Group Proteins
Globins
DNA