A natural outbreak of Newcastle disease (ND) was reported in a flock of guinea-fowl in Nigeria, affecting 1,029 birds of which 250 (24.3%) died. Paralysis of the legs and wings, coughing, sneezing, white diarrhoea and complete cessation of egg production were observed. Serum samples collected at the onset and during the course of the disease had high ND antibody titres. ND virus was isolated from a pool of brain tissues from diseased guinea-fowl. The ND virus isolate was characterised as a velogenic strain.