The chronic myelocytic cell line K 562 contains minor (m) as well as major (M) ber/abl fusion mRNAs

R Seelig; P Schranz; C Bottner; A Neubauer; H P Seelig; M Renz

The chronic myelocytic cell line K 562 contains minor (m) as well as major (M) ber/abl fusion mRNAs

Leukemia. 1993 Nov;7(11):1886-7.

Authors

R Seelig¹, P Schranz, C Bottner, A Neubauer, H P Seelig, M Renz

Affiliation

¹ Institute of Immunology and Molecular Genetics, Karlsruhe, Germany.

PMID: 8231257

Abstract

By searching for additional chimeric bcr/abl transcripts in K 562 cells characterized by major (M) bcr/abl fusions, a new mRNA, a minor (m) bcr/abl transcript, was detected. A practical implication of this finding is that the K 562 cell line can be used as positive control for the detection by the polymerase chain reaction of both types of transcripts for the diagnosis of Philadelphia chromosome associated leukemias.

MeSH terms

Base Sequence
Fusion Proteins, bcr-abl / genetics*
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
Molecular Sequence Data
Polymerase Chain Reaction / methods
RNA, Messenger / analysis*
RNA, Neoplasm / analysis*
Transcription, Genetic
Tumor Cells, Cultured / chemistry

Substances

RNA, Messenger
RNA, Neoplasm
Fusion Proteins, bcr-abl