Structure at 2.5 A of a designed peptide that maintains solubility of membrane proteins

Science. 1993 Oct 29;262(5134):734-8. doi: 10.1126/science.8235592.

Abstract

A 24-amino acid peptide designed to solubilize integral membrane proteins has been synthesized. The design was for an amphipathic alpha helix with a "flat" hydrophobic surface that would interact with a transmembrane protein as a detergent. When mixed with peptide, 85 percent of bacteriorhodopsin and 60 percent of rhodopsin remained in solution over a period of 2 days in their native forms. The crystal structure of peptide alone showed it to form an antiparallel four-helix bundle in which monomers interact, flat surface to flat surface, as predicted.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacteriorhodopsins / chemistry
  • Crystallography, X-Ray
  • Detergents / chemical synthesis
  • Detergents / chemistry*
  • Drug Design
  • Membrane Proteins / chemistry*
  • Models, Molecular
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / chemistry*
  • Protein Conformation
  • Protein Structure, Secondary
  • Rhodopsin / chemistry

Substances

  • Detergents
  • Membrane Proteins
  • Peptides
  • peptitergent PD1
  • Bacteriorhodopsins
  • Rhodopsin