Optimization of RNA and DNA purification procedures from human colorectal carcinomas

M La Farina; M Alberti; A Russo; V Bazan; G Grasso; D Romancino; P Bazan; I Albanese

Optimization of RNA and DNA purification procedures from human colorectal carcinomas

Anticancer Res. 1993 Sep-Oct;13(5A):1485-90.

Authors

M La Farina¹, M Alberti, A Russo, V Bazan, G Grasso, D Romancino, P Bazan, I Albanese

Affiliation

¹ Dipartimento di Biologia Cellulare e dello Sviluppo, University of Palermo, Italy.

PMID: 8239526

Abstract

We optimized procedures to extract RNA and DNA separately from colorectal carcinomas pulverized by Microdismembrator II. Our procedures make it possible to achieve good yields and high degree of purification of both types of macromolecules without affecting their structural integrity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorectal Neoplasms / chemistry*
DNA, Neoplasm / isolation & purification*
Electrophoresis
Humans
Nucleic Acid Hybridization
RNA, Neoplasm / isolation & purification*

Substances

DNA, Neoplasm
RNA, Neoplasm