Abstract
By the use of fura-2 and digital imaging techniques, [K]o depolarization or PAF (10(-9) M) were shown to induce a sustained increase of [Ca]i in human or canine single aortic vascular endothelial cells (VEC) that was insensitive to nifedipine but sensitive to (-)-PN200-110 or to lowering of [Ca]o. The PAF-induced effect on [Ca]i was blocked by the PAF receptor antagonist, WEB2170. Our results suggest that [K]o depolarization and PAF increase [Ca]i via the activation of R-type Ca2+ channels.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azepines / pharmacology
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Calcium Channel Blockers / pharmacology
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Calcium Channels / metabolism*
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Calcium Channels / physiology
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Electrophysiology
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Endothelium, Vascular / cytology
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Endothelium, Vascular / metabolism*
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Fura-2
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Humans
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Image Processing, Computer-Assisted
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In Vitro Techniques
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Ion Channel Gating / physiology*
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Isradipine / pharmacology
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Male
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Platelet Activating Factor / antagonists & inhibitors
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Platelet Activating Factor / metabolism*
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Potassium / pharmacology
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Triazoles / pharmacology
Substances
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Azepines
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Calcium Channel Blockers
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Calcium Channels
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Platelet Activating Factor
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Triazoles
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bepafant
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Potassium
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Fura-2
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Isradipine