Abstract
A novel serine kinase, named nuclear factor kappa B (NF kappa B) kinase, has been shown to be associated with the NF kappa B.I kappa B complex in the cytosol of human primary T lymphocytes. It activates the DNA binding activity of NF kappa B by directly phosphorylating its p65 and p50 subunits. There is no evidence that it phosphorylates I kappa B. Experiments with inhibitors and antisera showed that it is distinct from other known serine kinases such as mitogen-activated protein kinase or Mos. It has an apparent molecular size of 43 kDa determined by the substrate binding assay. This kinase might have a central role in mediating various signals to NF kappa B.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Base Sequence
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Cells, Cultured
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Cytosol / metabolism
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DNA / metabolism
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DNA-Binding Proteins / metabolism
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Humans
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Molecular Sequence Data
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NF-kappa B / metabolism*
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NF-kappaB-Inducing Kinase
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Phosphorylation
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Precipitin Tests
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Protein Serine-Threonine Kinases / metabolism*
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Proto-Oncogene Proteins / metabolism
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Substrate Specificity
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T-Lymphocytes / enzymology
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T-Lymphocytes / metabolism
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Transcription Factor RelB
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Transcription Factors*
Substances
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DNA-Binding Proteins
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NF-kappa B
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Proto-Oncogene Proteins
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RELB protein, human
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Transcription Factors
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Transcription Factor RelB
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Adenosine Triphosphate
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DNA
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Protein Serine-Threonine Kinases