(1,3)-beta-D-Glucanase isoenzyme GII and (1,3;1,4)-beta-D-glucanase isoenzyme EII from barley have been crystallized by the hanging drop method in the presence of ammonium sulphate. The crystals of the (1,3)-beta-D-glucanase, which diffract to about 1.8 A resolution, belong to the trigonal space group P3(1)2(1)2 (or P3(2)2(1)2) with cell constants a = b = 86.9 A, c = 156.0 A and contain two molecules in the asymmetric unit. The crystals of the (1,3;1,4)-beta-D-glucanase which diffract to better than 1.8 A resolution, belong to the tetragonal space group P4(3)2(1)2 (or P4(1)2(1)2) with cell constants a = b = 87.4 A, c = 109.5 A and contain one molecule in the asymmetric unit.