Regional localization of the lactase-phlorizin hydrolase gene, LCT, to chromosome 2q21

Ann Hum Genet. 1993 Jul;57(3):179-85. doi: 10.1111/j.1469-1809.1993.tb01593.x.

Abstract

The gene LCT which codes for the intestinal disaccharidase lactase-phlorizin hydrolase has previously been mapped, using somatic cell hybrids and linkage analysis, using the CEPH families, to chromosome 2. We describe here the regional localization of LCT to chromosome 2q21 by polymerase chain reaction (PCR) analysis of somatic cell hybrids and in situ hybridization. LCT is closely linked to D2S44, with a lod score of 30.6 at theta = 0.10.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 2*
  • DNA Primers
  • Exons
  • Female
  • Genetic Markers
  • Humans
  • Hybrid Cells
  • Karyotyping
  • Lactase-Phlorizin Hydrolase / genetics*
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Rodentia

Substances

  • DNA Primers
  • Genetic Markers
  • Lactase-Phlorizin Hydrolase