Abstract
The structure of the glycan moiety of the glycosyl-phosphatidylinositol (GPI) membrane anchor from Torpedo californica (electric fish) electric-organ acetylcholinesterase was solved using n.m.r., methylation analysis and chemical and enzymic micro-sequencing. Two structures were found to be present: Glc alpha 1-2Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol and Glc alpha 1-2Man alpha 1-2Man alpha 1-6(GalNAc beta 1-4)Man alpha 1-4GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholinesterase / chemistry*
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Acetylcholinesterase / isolation & purification
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Animals
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Carbohydrate Conformation
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Carbohydrate Sequence
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Cell Membrane / enzymology
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Electric Organ / enzymology*
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Glycoside Hydrolases
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Glycosylphosphatidylinositols / chemistry*
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Glycosylphosphatidylinositols / isolation & purification
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Magnetic Resonance Spectroscopy
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Methylation
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Molecular Sequence Data
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Oligosaccharides / chemistry
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Oligosaccharides / isolation & purification
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Phosphatidylinositol Diacylglycerol-Lyase
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Phosphoric Diester Hydrolases / metabolism
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Torpedo
Substances
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Glycosylphosphatidylinositols
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Oligosaccharides
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Acetylcholinesterase
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Phosphoric Diester Hydrolases
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Glycoside Hydrolases
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Phosphatidylinositol Diacylglycerol-Lyase