Abstract
A transcription map covering a 300 kb region around the G6PD gene in the human Xq28 region was constructed by the direct cDNA selection method and the analysis of the resulting region-specific enriched cDNA sublibrary. Seven new genes and two loci of endogenous retrovirus HERV-K were identified. The distribution of the genes across the region is strongly non-uniform and follows the non-uniform distribution of GpG islands in the area. While one of the novel genes was found to be highly homologous to bovine smg p25A GDP-dissociation inhibitor, the remaining genes did not detect any homology to known genes. The analysis of region-specific cDNA sublibraries represents a simple, rapid and efficient tool for the generation of a regional transcription map.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Chromosome Mapping
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DNA, Complementary / analysis*
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Dinucleoside Phosphates / analysis
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GTP-Binding Proteins / genetics
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Gene Library
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Glucosephosphate Dehydrogenase / biosynthesis
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Glucosephosphate Dehydrogenase / genetics*
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Humans
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Molecular Sequence Data
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Organ Specificity
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Restriction Mapping
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Retroviridae / genetics
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Sequence Homology, Nucleic Acid
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Transcription, Genetic*
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X Chromosome*
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rab3 GTP-Binding Proteins
Substances
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DNA, Complementary
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Dinucleoside Phosphates
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cytidylyl-3'-5'-guanosine
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Glucosephosphate Dehydrogenase
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GTP-Binding Proteins
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rab3 GTP-Binding Proteins
Associated data
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GENBANK/X74605
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GENBANK/X74606
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GENBANK/X74607
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GENBANK/X74608
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GENBANK/X74609
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GENBANK/X74610
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GENBANK/X74611
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GENBANK/X74612
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GENBANK/X74613