Expression and function of an IgE-binding animal lectin (epsilon BP) in mast cells

Immunopharmacology. 1993 Nov-Dec;26(3):187-95. doi: 10.1016/0162-3109(93)90034-n.

Abstract

epsilon BP (IgE-binding protein) is a 31,000 M(r) protein originally identified in rat basophilic leukemia (RBL) cells. The protein is composed of two domains with the amino-terminal domain containing a highly conserved repetitive sequence and the carboxyl-terminal domain containing consensus sequences shared by other beta-galactoside-binding soluble lectins. The protein has wide tissue distribution, is found on cell surfaces and in extracellular milieu. By combined efforts from several research groups including ours a multifunctional nature of this lectin began to emerge. This review emphasizes the following characteristics of epsilon BP: (i) epsilon BP is secreted by cells such as macrophages; (ii) like many other lectins, epsilon BP functions at least bivalently; (iii) epsilon BP has specificity for distinct oligosaccharide structures that have a terminal galactose not masked by sialic acids; and (iv) in addition to binding IgE, epsilon BP binds to surfaces of various cell types via lectin-carbohydrate interaction. Importantly, epsilon BP binds to the IgE receptor on mast cells. We propose that epsilon BP can function as a modulatory protein on various cells by cross-linking critical cell surface glycoproteins. The proposed action of epsilon BP on mast cells is presented as a model.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation / biosynthesis
  • Antigens, Differentiation / chemistry
  • Antigens, Differentiation / physiology*
  • Binding Sites
  • Electrophoresis, Polyacrylamide Gel
  • Galectin 3
  • Lectins / biosynthesis
  • Lectins / chemistry
  • Lectins / physiology*
  • Macrophages / immunology
  • Mast Cells / immunology*
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Receptors, IgE / metabolism*

Substances

  • Antigens, Differentiation
  • Galectin 3
  • Lectins
  • Membrane Glycoproteins
  • Receptors, IgE