Identification of amino acid-base contacts in the Myc-DNA complex by site-specific bromouracil mediated photocrosslinking

Q Dong; E E Blatter; Y W Ebright; K Bister; R H Ebright

doi:10.1002/j.1460-2075.1994.tb06249.x

Identification of amino acid-base contacts in the Myc-DNA complex by site-specific bromouracil mediated photocrosslinking

EMBO J. 1994 Jan 1;13(1):200-4. doi: 10.1002/j.1460-2075.1994.tb06249.x.

Authors

Q Dong¹, E E Blatter, Y W Ebright, K Bister, R H Ebright

Affiliation

¹ Department of Chemistry, Rutgers University, New Brunswick, NJ 08855.

Abstract

Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C-3A-2C-1G+1T+2G+3-3'. Using site-specific 5-bromouracil mediated photocrosslinking, we show that His336 of Myc contacts, or is close to, the thymine 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc-DNA complex. Our results strongly suggest that homologous amino acids of Myc and Max make equivalent contacts in the respective protein-DNA complexes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acids / metabolism
Base Sequence
Binding Sites
Bromouracil / pharmacology
Cross-Linking Reagents / pharmacology
Crystallography, X-Ray
DNA / chemistry
DNA / metabolism*
Molecular Conformation
Molecular Sequence Data
Nucleotides / metabolism
Proto-Oncogene Proteins c-myc / chemistry
Proto-Oncogene Proteins c-myc / metabolism*
Sequence Homology, Amino Acid

Substances

Amino Acids
Cross-Linking Reagents
Nucleotides
Proto-Oncogene Proteins c-myc
Bromouracil
DNA