Discrimination of epidemic and sporadic isolates of Arcobacter butzleri by polymerase chain reaction-mediated DNA fingerprinting

J Clin Microbiol. 1993 Dec;31(12):3317-9. doi: 10.1128/jcm.31.12.3317-3319.1993.

Abstract

DNA polymorphisms of Arcobacter butzleri outbreak-related strains and Arcobacter reference strains were determined by use of the polymerase chain reaction with primers aimed at repetitive sequences. The epidemiological relationship among 14 outbreak-related strains was substantiated, as they showed virtually no genomic variations. Their DNA amplification patterns were, however, clearly different from those of all Arcobacter reference strains studied; each reference strain was characterized by a unique DNA fingerprint.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Campylobacter / classification
  • Campylobacter / genetics*
  • Campylobacter / isolation & purification*
  • Campylobacter Infections / epidemiology
  • Campylobacter Infections / microbiology*
  • Child
  • Child, Preschool
  • DNA Fingerprinting
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Disease Outbreaks
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Repetitive Sequences, Nucleic Acid
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Bacterial