Abstract
A novel genetic selection in yeast has been used to isolate a complementary DNA for the transcriptional activator, Olf-1, which binds to the regulatory sequences of several olfactory-specific genes. The Olf-1 protein, expressed exclusively in the olfactory receptor neurons and their precursors, contains a new helix-loop-helix motif and functions as an apparent homodimer. Olf-1 may be the first member of a family of related proteins that may direct cellular differentiation in a variety of neuronal tissues.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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DNA-Binding Proteins / genetics*
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Genetic Vectors
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Neurons, Afferent / chemistry*
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Olfactory Mucosa / innervation
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Olfactory Pathways / chemistry*
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Plasmids
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Protein Structure, Secondary
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Rats
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Rats, Sprague-Dawley
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Recombinant Fusion Proteins
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Regulatory Sequences, Nucleic Acid
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Amino Acid
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Trans-Activators*
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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Ebf1 protein, rat
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Nerve Tissue Proteins
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Recombinant Fusion Proteins
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Trans-Activators
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Transcription Factors