Abstract
The structure of the Vibrio mimicus W-26768 O-antigen polysaccharide has been investigated by sugar and methylation analyses, Smith degradation, and NMR spectroscopy. It is proposed that it is composed of chains of beta-(1-->4)-linked 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucopyranosyl residues (D-Qui(p)3NR), approximately 60% of which are substituted in the 2-position with 2-acetamido-2-deoxy-alpha-D-galactopyranosyl groups. The polysaccharide does not seem to be composed of oligosaccharide repeating units but has a less regular structure, schematically indicated below. [formula: see text]
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Conformation
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Carbohydrate Sequence
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Diarrhea / microbiology
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Humans
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Indicators and Reagents
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Lipopolysaccharides / chemistry*
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Lipopolysaccharides / isolation & purification
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Magnetic Resonance Spectroscopy
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Molecular Sequence Data
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Oligosaccharides / chemistry
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Oligosaccharides / isolation & purification
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Polysaccharides, Bacterial / chemistry*
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Polysaccharides, Bacterial / isolation & purification
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Vibrio / chemistry*
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Vibrio / isolation & purification
Substances
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Indicators and Reagents
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Lipopolysaccharides
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Oligosaccharides
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Polysaccharides, Bacterial