Comparison of techniques for detecting antigens of Giardia lamblia and Cryptosporidium parvum in faeces

J Clin Pathol. 1993 Jun;46(6):555-8. doi: 10.1136/jcp.46.6.555.

Abstract

Aim: To compare the use of commercial monoclonal antibody test systems--the Giardia CEL IF test and the Crypto CEL IF test--for the detection of Giardia lamblia and Cryptosporidium parvum antigens in faeces with conventional techniques.

Methods: Sensitivity and specificity were evaluated using preparations of cysts of G lamblia and purified oocysts of C parvum. Evaluation of 59 random faecal samples passing through the Department of Clinical Parasitology, Hospital for Tropical Diseases, London, was carried out for both organisms.

Results: The fluorescence staining techniques proved more sensitive than other tests routinely used for diagnosis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Protozoan / analysis*
  • Cryptosporidiosis / diagnosis*
  • Cryptosporidium parvum / immunology*
  • Feces / parasitology*
  • Fluorescent Antibody Technique
  • Giardia lamblia / immunology*
  • Giardiasis / diagnosis*
  • Humans
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Antigens, Protozoan
  • Reagent Kits, Diagnostic