Abstract
We have identified a molecule expressed by human marrow granulocyte/monocyte colony-forming cells (CFU-GM), erythroid colony-forming cells (CFU-E), and erythroid burst-forming units (BFU-E), but not their precursors detectable in long-term bone marrow culture. This antigen, detected by flow microfluorimetry using monoclonal antibody 7B9, is coexpressed with CD33 on many CD34+ CFCs, although only the 7B9 antigen was detected on a portion of BFU-E and CFU-E, whereas only CD33 was found on a portion of CFU-GM. Antibody 7B9 appears to be useful for isolating subsets of progenitors based on their common or selective expression of 7B9 antigen and CD33.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal / immunology
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Antigens, CD / analysis*
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Antigens, Differentiation, Myelomonocytic / analysis*
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Antigens, Surface / analysis*
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Bone Marrow Cells*
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Erythroid Precursor Cells / cytology*
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Erythroid Precursor Cells / immunology
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Flow Cytometry
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Fluorescent Antibody Technique
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Hematopoiesis*
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Hematopoietic Stem Cells / cytology*
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Hematopoietic Stem Cells / immunology
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Humans
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In Vitro Techniques
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Molecular Weight
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Sialic Acid Binding Ig-like Lectin 3
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Time Factors
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation, Myelomonocytic
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Antigens, Surface
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CD33 protein, human
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Sialic Acid Binding Ig-like Lectin 3