Polyphasic taxonomy leading to the proposal of Moraxella canis sp. nov. for Moraxella catarrhalis-like strains

Int J Syst Bacteriol. 1993 Jul;43(3):438-49. doi: 10.1099/00207713-43-3-438.

Abstract

The taxonomic position of a group of 16 Moraxella catarrhalis-like strains, isolated mainly from dogs, was examined by using morphological tests, biochemical tests, serology, ribotyping with oligonucleotide probes, polymerase chain reaction typing of the 16S rRNA gene and the 16S-23S rRNA gene spacer region, polyacrylamide gel electrophoresis of total proteins, fatty acid profiles, moles percent G+C, dot spot and in-solution DNA-DNA hybridizations, and DNA-rRNA hybridizations. It was found that these organisms constitute a distinct cluster within the genus Moraxella. Since they differ genotypically as well as phenotypically from previously described Moraxella species, a new species, Moraxella canis, is proposed to accommodate these isolates. The type strain is LMG 11194 (= N7 = CCUG 8415A).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / analysis
  • Bacterial Typing Techniques
  • Base Composition
  • Base Sequence
  • Cats / microbiology
  • Cluster Analysis
  • Cross Reactions
  • DNA, Ribosomal / genetics
  • Dogs / microbiology*
  • Fatty Acids / analysis
  • Lipopolysaccharides / immunology
  • Molecular Sequence Data
  • Moraxella / classification*
  • Moraxella / genetics*
  • Moraxella / isolation & purification
  • Moraxella catarrhalis / classification
  • Moraxella catarrhalis / genetics
  • Neisseria meningitidis / immunology
  • Nucleic Acid Hybridization
  • Respiratory Tract Infections / microbiology
  • Serotyping

Substances

  • Bacterial Proteins
  • DNA, Ribosomal
  • Fatty Acids
  • Lipopolysaccharides