Abstract
Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potentially be useful for demonstrating other medically important bacteria.
MeSH terms
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Antigens, Fungal
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Evaluation Studies as Topic
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Francisella tularensis / immunology
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Francisella tularensis / isolation & purification
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Francisella tularensis / ultrastructure*
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Gram-Negative Bacteria / classification
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Gram-Negative Bacteria / immunology
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Gram-Negative Bacteria / ultrastructure
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Humans
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Immunohistochemistry
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Microscopy, Immunoelectron / methods*
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Species Specificity
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Tularemia / diagnosis