The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region

A Di Donato; V Cafaro; M de Nigris; M Rizzo; G D'Alessio

doi:10.1006/bbrc.1993.1986

The determinants of the dimeric structure of seminal ribonuclease are located in its N-terminal region

Biochem Biophys Res Commun. 1993 Aug 16;194(3):1440-5. doi: 10.1006/bbrc.1993.1986.

Authors

A Di Donato¹, V Cafaro, M de Nigris, M Rizzo, G D'Alessio

Affiliation

¹ Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Italy.

PMID: 8352802
DOI: 10.1006/bbrc.1993.1986

Abstract

A chimeric cDNA was constructed, coding for the N-terminal region of BS-RNase (residues 1-49) and the C-terminal region of RNase A (residues 50-124). The resulting chimeric DNA was expressed in Escherichia coli and found to code for RNase chains that spontaneously assembled into a covalently dimeric ribonuclease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Protein Conformation*
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / chemistry*
Ribonuclease, Pancreatic / chemistry
Ribonuclease, Pancreatic / genetics
Ribonucleases / biosynthesis
Ribonucleases / chemistry*
Ribonucleases / genetics
Semen / enzymology*

Substances

Recombinant Fusion Proteins
Ribonucleases
Ribonuclease, Pancreatic