Non-injection methods for the production of embryonic stem cell-embryo chimaeras

Nature. 1993 Sep 2;365(6441):87-9. doi: 10.1038/365087a0.

Abstract

The simple aggregation of pluripotent ES cells with morulae-stage embryos can result in viable chimaeras at a similar frequency to that of blastocyst injection. The savings in time and equipment inherent in the aggregation techniques, however, make this approach well worth considering.

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Cell Aggregation
  • Cells, Cultured
  • Chimera*
  • Culture Techniques
  • Embryo, Mammalian / cytology*
  • Embryology / methods
  • Mice
  • Mice, Inbred C57BL
  • Microinjections
  • Morula / cytology
  • Stem Cells / cytology*