Immunogenicity in rabbits and monkeys of influenza ISCOMs conjugated with repeated sequences of the Plasmodium falciparum antigen Pf155/RESA

Parasite Immunol. 1993 Jun;15(6):355-9. doi: 10.1111/j.1365-3024.1993.tb00620.x.

Abstract

Rabbits and monkeys were immunized with two fusion proteins, ZZ-M3 and ZZ-M5, coupled to pre-formed influenza virus membrane glycoprotein ISCOMs. The fusion proteins comprise two IgG-binding domains from staphylococcal protein A (ZZ) and repeated amino acid sequences from the C-terminal (M3) or central (M5) repeat regions of the Plasmodium falciparum antigen Pf155/RESA. The induced antibody responses were of long duration, could be efficiently boosted and were comparable to those obtained with Freund's Adjuvant. The produced antibodies reacted with M3, M5, protein A and the influenza glycoprotein, recognized Pf155/RESA and inhibited merozoite invasion in vitro. These results suggest that coupling of immunogens to pre-formed ISCOMs may be a basis for construction of multivalent subunit vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Protozoan / biosynthesis
  • Antigens, Protozoan / immunology*
  • Antigens, Surface / immunology*
  • Bacterial Proteins / immunology
  • Base Sequence
  • Chlorocebus aethiops
  • Dose-Response Relationship, Immunologic
  • ISCOMs / chemistry
  • ISCOMs / immunology*
  • Immunization, Secondary
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / immunology
  • Influenza A virus / immunology*
  • Malaria, Falciparum / immunology
  • Malaria, Falciparum / prevention & control
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology
  • Plasmodium falciparum / immunology*
  • Protozoan Proteins / immunology*
  • Protozoan Vaccines / immunology*
  • Protozoan Vaccines / therapeutic use
  • Rabbits
  • Staphylococcal Protein A / immunology
  • Streptococcus / immunology
  • Time Factors
  • Vaccination
  • Vaccines, Synthetic / biosynthesis
  • Vaccines, Synthetic / immunology

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Antigens, Surface
  • Bacterial Proteins
  • ISCOMs
  • IgG Fc-binding protein, Streptococcus
  • Immunoglobulin G
  • Peptide Fragments
  • Protozoan Proteins
  • Protozoan Vaccines
  • Staphylococcal Protein A
  • Vaccines, Synthetic
  • ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum