Abstract
In situ hybridization for the norepinephrine transporter (NET) mRNA was performed in animals receiving short-term (2 days) and long-term (4 weeks) treatment with desipramine (DMI; 10 mg/kg, intraperitoneal). Following short-term and long-term DMI treatment, a significant (P < 0.05) increase in hybridization of 35S-labeled oligonucleotides to NET mRNA in the locus coeruleus was observed compared to that observed in vehicle-treated animals. The mechanism of this increase in transporter mRNA or its involvement in the therapeutic effects of anti-depressants remains to be determined.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carrier Proteins / biosynthesis*
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Desipramine / pharmacology*
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Image Processing, Computer-Assisted
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In Situ Hybridization
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Locus Coeruleus / drug effects
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Locus Coeruleus / metabolism*
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Male
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Norepinephrine / metabolism*
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Norepinephrine Plasma Membrane Transport Proteins
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Oligonucleotide Probes / chemical synthesis
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Oligonucleotide Probes / pharmacology
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RNA, Messenger / biosynthesis*
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Rats
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Rats, Sprague-Dawley
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Stimulation, Chemical
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Sulfur Radioisotopes
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Symporters*
Substances
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Carrier Proteins
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Norepinephrine Plasma Membrane Transport Proteins
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Oligonucleotide Probes
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RNA, Messenger
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Slc6a2 protein, rat
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Sulfur Radioisotopes
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Symporters
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Desipramine
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Norepinephrine