Crystallization and preliminary crystallographic analysis of a TNF-beta-55 kDa TNF receptor complex

J Mol Biol. 1993 Jan 20;229(2):555-7. doi: 10.1006/jmbi.1993.1055.

Abstract

A complex of tumour necrosis factor-beta with the soluble extracellular domain of the human 55 kDa TNF receptor has been crystallized. Crystals of the complex were grown using polyethylene glycol 4000 as the precipitating agent in the presence of beta-octyl glucoside. The receptor-ligand complex crystallizes in a cubic space group and diffracts to 2.85 A.

MeSH terms

  • Crystallization
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Lymphotoxin-alpha / chemistry*
  • Lymphotoxin-alpha / metabolism
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / metabolism
  • Receptors, Tumor Necrosis Factor
  • X-Ray Diffraction

Substances

  • Lymphotoxin-alpha
  • Receptors, Cell Surface
  • Receptors, Tumor Necrosis Factor