Abstract
Bovine seminal ribonuclease, a uniquely dimeric pancreatic-like RNase, with its dimeric structure stabilized by two intersubunit disulfides, and endowed with special, i.e. non-catalytic, actions (antitumor, immunosuppressive, antispermatogenic), was stably expressed in Chinese hamster ovary cells. The recombinant protein, secreted in the culture medium as a correctly folded dimeric enzyme, was purified to homogeneity and found to be fully active both in its catalytic and antitumor activities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells / drug effects
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Animals
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CHO Cells / metabolism
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Cattle
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Cell Division / drug effects
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Cell Line, Transformed
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Chromatography, Ion Exchange
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Cricetinae
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Electrophoresis, Polyacrylamide Gel
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Gene Expression*
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Macromolecular Substances
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Male
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Mice
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Ribonucleases / chemistry
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Ribonucleases / genetics*
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Ribonucleases / pharmacology
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Seminal Vesicles / enzymology*
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Simian virus 40
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Transfection
Substances
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Macromolecular Substances
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Recombinant Proteins
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Ribonucleases