Abstract
The IgG and IgM immunoblotting patterns for the major HCMV antigens p65 and p55/52 were studied in 5 renal transplant recipients during primary HCMV infections. Sequential sera from the 5 patients were tested in parallel with standardized antigens derived from the reference strain AD169 and from the patient'own isolates. The immunoblotting patterns differed from patient to patient as well as between strain AD169 and the patient's homologous isolates. The differences consisted in a better response to the antigens derived from the reference strain AD169, suggesting a delay of the IgM/IgG switch and of the affinity maturation of IgG antibodies to the homologous p65 and p55/52 antigens. The results suggest caution in the interpretation of immunoblotting data derived from laboratory strains such as AD169.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antibodies, Viral / biosynthesis
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Antibody Affinity
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Antigenic Variation
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Antigens, Viral / immunology*
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Antigens, Viral / isolation & purification
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Child
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Cytomegalovirus / immunology*
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Cytomegalovirus Infections / diagnosis
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Cytomegalovirus Infections / etiology
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Cytomegalovirus Infections / immunology*
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Female
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Humans
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Immunoblotting
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Immunoglobulin G / biosynthesis*
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Immunoglobulin M / biosynthesis*
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Kidney Transplantation / adverse effects*
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Male
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Middle Aged
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Phosphoproteins / immunology*
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Phosphoproteins / isolation & purification
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Viral Matrix Proteins / immunology*
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Viral Matrix Proteins / isolation & purification
Substances
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Antibodies, Viral
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Antigens, Viral
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Immunoglobulin G
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Immunoglobulin M
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Phosphoproteins
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Viral Matrix Proteins
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cytomegalovirus matrix protein 65kDa
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human cytomegalovirus antigen p55-52