[In vivo identification of YB-1 protein, interacting with the enhancer of human papillomavirus (HPV) type 18, using antibodies to a synthetic peptide]

Mol Biol (Mosk). 1993 Jan-Feb;27(1):81-91.
[Article in Russian]

Abstract

Enhancer sequences of human papilloma virus (HPV) type 18 were used for screening of HeLa cells cDNA library in lambda gt11 using the protein binding method. Clones with YB I gene homology sequences were isolated. This gene is coding the protein which binds the regulatory region of Y gene of main histocompatibility complex (HLA 11). The YB I transcripts were revealed in all tested samples of cervical carcinomas. To analyze the protein the rabbit antibodies were produced to synthetic peptide, which corresponds to the most hydrophilic region of the protein. This antipeptide serum allowed to identify the nuclear 42K protein in HeLa cells as well as in normal fibroblasts.

Publication types

  • English Abstract

MeSH terms

  • Amino Acid Sequence
  • Antibodies / immunology*
  • Base Sequence
  • Blotting, Western
  • CCAAT-Enhancer-Binding Proteins*
  • Cells, Cultured
  • DNA
  • DNA-Binding Proteins / genetics*
  • Enhancer Elements, Genetic*
  • Genes, Viral
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Nuclear Proteins
  • Oncogene Proteins, Viral / genetics
  • Papillomaviridae / genetics*
  • Peptides / immunology*
  • Transcription Factors*
  • Y-Box-Binding Protein 1

Substances

  • Antibodies
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • E6 protein, Human papillomavirus type 18
  • NFI Transcription Factors
  • Nuclear Proteins
  • Oncogene Proteins, Viral
  • Peptides
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human
  • DNA