M13 and pUC vectors with new unique restriction sites for cloning

Gene. 1993 Aug 16;130(1):151-2. doi: 10.1016/0378-1119(93)90360-f.

Abstract

Several vectors based on the widely used phage M13 and plasmid pUC were constructed. The vectors contain polylinkers (MCS) for DNA insertions with several new restriction sites (e.g., ApaI, NotI, StuI, SacII). Moreover, the NarI site in the nonessential part of the vector molecule was changed into a BssHII site, so that the NarI site in the MCS became unique.

MeSH terms

  • Amino Acid Sequence
  • Bacteriophage M13 / genetics*
  • Base Sequence
  • Cloning, Molecular / methods*
  • DNA Restriction Enzymes
  • DNA, Recombinant / analysis
  • Deoxyribonucleases, Type II Site-Specific
  • Genetic Vectors*
  • Molecular Sequence Data
  • Mutagenesis, Insertional / methods
  • Mutagenesis, Site-Directed
  • Oligodeoxyribonucleotides
  • Plasmids / genetics*
  • Restriction Mapping*

Substances

  • DNA, Recombinant
  • Oligodeoxyribonucleotides
  • DNA Restriction Enzymes
  • endodeoxyribonuclease BSSHII
  • Deoxyribonucleases, Type II Site-Specific
  • GGCGCC-specific type II deoxyribonucleases

Associated data

  • GENBANK/L03653
  • GENBANK/L03654
  • GENBANK/L07632
  • GENBANK/M96932
  • GENBANK/M96933
  • GENBANK/S65683
  • GENBANK/S65686
  • GENBANK/S65693
  • GENBANK/S65694
  • GENBANK/X54209