Abstract
An effective HIV vaccine should be capable of eliciting virus-specific cytotoxic T lymphocytes (CTL). We have characterized the cellular and molecular features of a simian immunodeficiency virus of macaques (SIVmac) gag-specific CTL response in rhesus monkeys. We have shown that SIVmac-infected rhesus monkeys expressing the major histocompatibility complex (MHC) class I molecule Mamu-A*01 develop a SIVmac gag-specific CTL response which recognizes a 9 amino acid fragment of the gag protein in association with Mamu-A*01. Moreover, this peptide/MHC class I recognition is mediated by T cell receptors (TCR) employing a predominant V beta gene family and J beta gene. Using this understanding of a SIVmac-specific CTL response, we have shown that SIVmac-specific CTL can be elicited through three novel approaches to vaccination: a recombinant viral vector, a recombinant bacterial vector and a peptide vaccine. These studies illustrate the utility of the SIV/macaque model in AIDS vaccine research.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Gene Products, env / immunology
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Histocompatibility Antigens Class I / immunology
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Liposomes
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Macaca mulatta / immunology*
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Mycobacterium bovis / genetics
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Mycobacterium bovis / immunology
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Parainfluenza Virus 1, Human / immunology
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Peptide Fragments / immunology
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology
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Retroviridae Proteins, Oncogenic / immunology
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Simian Acquired Immunodeficiency Syndrome / immunology*
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Simian Acquired Immunodeficiency Syndrome / pathology
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Simian Immunodeficiency Virus / immunology*
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / pathology
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Vaccination
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Vaccines, Synthetic / immunology
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Vaccinia virus / genetics
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Viral Fusion Proteins*
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Viral Vaccines*
Substances
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Gene Products, env
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Histocompatibility Antigens Class I
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Liposomes
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Peptide Fragments
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Receptors, Antigen, T-Cell
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Retroviridae Proteins, Oncogenic
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Vaccines, Synthetic
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Viral Fusion Proteins
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Viral Vaccines
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transmembrane protein, Simian immunodeficiency virus