Bromodeoxyuridine-labelled apoptosis after treatment with antimetabolites in two murine tumours and in small intestinal crypts

C E Sarraf; T W Ansari; P Conway; M Notay; S Hill; M R Alison

doi:10.1038/bjc.1993.408

Bromodeoxyuridine-labelled apoptosis after treatment with antimetabolites in two murine tumours and in small intestinal crypts

Br J Cancer. 1993 Oct;68(4):678-80. doi: 10.1038/bjc.1993.408.

Authors

C E Sarraf¹, T W Ansari, P Conway, M Notay, S Hill, M R Alison

Affiliation

¹ Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.

Abstract

Antimetabolites are S-phase specific anticancer drugs. Administration of bromodeoxyuridine (BrdUrd) to tumour bearing mice was followed by treatment with cytosine arabinoside or hydroxyurea. Anti-BrdUrd immunocytochemistry visualised susceptible tumour and intestinal crypt cells at electron microscope level, showing unequivocally that cells that were in S-phase at the time of administration of the drugs subsequently died by apoptosis.

MeSH terms

Animals
Apoptosis / drug effects*
Apoptosis / physiology
Bromodeoxyuridine
Carcinoma / drug therapy
Carcinoma / pathology*
Cytarabine / pharmacology*
Hydroxyurea / pharmacology*
Intestine, Small / drug effects
Intestine, Small / pathology*
Male
Mice
Mice, Inbred CBA
Mitotic Index / drug effects
S Phase*
Sarcoma, Experimental / drug therapy
Sarcoma, Experimental / pathology*

Substances

Cytarabine
Bromodeoxyuridine
Hydroxyurea