Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I

Gene. 1993 Sep 15;131(2):287-92. doi: 10.1016/0378-1119(93)90308-p.

Abstract

We have cloned and sequenced the human cardiac troponin I (cTnI)-encoding cDNA with the aim of expressing the cDNA in Escherichia coli. The cDNA was successfully expressed as a fusion product with beta-galactosidase and as an unfused protein. Both polypeptides were recognised by an anti-human cTnI antibody.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary
  • Escherichia coli
  • Humans
  • Molecular Sequence Data
  • Myocardium / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Sequence Homology, Amino Acid
  • Troponin / genetics*
  • Troponin I
  • beta-Galactosidase / genetics

Substances

  • DNA, Complementary
  • Recombinant Fusion Proteins
  • Troponin
  • Troponin I
  • beta-Galactosidase

Associated data

  • GENBANK/L24502
  • GENBANK/L24522
  • GENBANK/L24523
  • GENBANK/L24524
  • GENBANK/L24525
  • GENBANK/L24526
  • GENBANK/L24527
  • GENBANK/L24528
  • GENBANK/L24529
  • GENBANK/M64247
  • PIR/A33185