Genes required for extracellular secretion of enterotoxin are clustered in Vibrio cholerae

Gene. 1993 Sep 30;132(1):101-6. doi: 10.1016/0378-1119(93)90520-d.

Abstract

Pleiotropic transposon insertion mutants of Vibrio cholerae that are unable to secrete enterotoxin, HA/protease and chitinase through the outer membrane have been isolated. The gene, epsM, responsible for complementation of two of the Tn5 insertion mutations was sequenced. It encodes a putative cytoplasmic membrane protein of 18.5 kDa that exhibits similarity to proteins required for extracellular secretion of pullulanase, pectate lyase or elastase in other Gram-bacteria. It is present on a 15-kb DNA fragment from the V. cholerae genome, containing the epsE gene that was previously shown to be required for secretion of cholera toxin [Sandkvist et al., Gene 123 (1993) 81-86]. Partial reading frames flanking epsM also demonstrated similarity to genes required for extracellular secretion of pullulanase in Klebsiella oxytoca.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • DNA Transposable Elements
  • Deoxyribonucleases, Type II Site-Specific
  • Enterotoxins / metabolism*
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Membrane Proteins*
  • Molecular Sequence Data
  • Multigene Family*
  • Peptides / genetics
  • Sequence Homology, Amino Acid
  • Vibrio cholerae / genetics*

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Enterotoxins
  • EpsE protein, Vibrio cholerae
  • EpsM protein, Vibrio cholerae
  • Membrane Proteins
  • Peptides
  • stN protein, Vibrio cholerae
  • Deoxyribonucleases, Type II Site-Specific
  • GGTACC-specific type II deoxyribonucleases

Associated data

  • GENBANK/L13267
  • GENBANK/L13268
  • GENBANK/L13660
  • GENBANK/L24523
  • GENBANK/L24524
  • GENBANK/L24525
  • GENBANK/L24526
  • GENBANK/L24527
  • GENBANK/L24528
  • GENBANK/L24529